The application of masks varies depending on the type:
Physical Masks: These are manually placed over the sample. For example, a stencil might be used during staining to prevent certain areas from being exposed to the stain. Digital Masks: These are created using image analysis software. Researchers can define regions of interest (ROI) and apply the mask to isolate these areas for further analysis. Optical Masks: These are used in conjunction with microscopy equipment to control the light path and enhance imaging.
