Detection of IEGs in histological samples can be achieved through various techniques. Common methods include:
Immunohistochemistry (IHC): This technique uses antibodies to detect IEG proteins within tissue sections. In situ hybridization (ISH): This method involves using labeled complementary RNA or DNA probes to detect IEG mRNA within tissues. RT-qPCR: Reverse transcription quantitative PCR is used to quantify IEG mRNA levels in tissue extracts. Western blotting: This technique is used to detect and quantify IEG proteins in tissue lysates.
