Creating histology datasets involves several steps:
Sample Collection: Biological tissues are collected from patients or experimental subjects. Fixation and Embedding: Tissues are preserved using chemical fixatives and embedded in a medium like paraffin to maintain their structure. Sectioning: Thin sections of the embedded tissues are cut using a microtome. Staining: Sections are stained with dyes such as hematoxylin and eosin (H&E) to enhance contrast and highlight different tissue components. Imaging: Stained sections are imaged using a microscope, and the images are digitized. Annotation and Labeling: Expert pathologists annotate the images with relevant labels, identifying structures or pathological features.