Collecting histological samples from pigs involves several key steps:
Euthanasia: Pigs are humanely euthanized following ethical guidelines to minimize suffering. Tissue Fixation: Tissues are immediately fixed in formaldehyde or other fixatives to preserve cellular architecture and prevent degradation. Tissue Processing: Fixed tissues are dehydrated, cleared, and embedded in paraffin wax to create solid blocks for sectioning. Microtomy: Thin sections (3-5 µm) are cut from the paraffin blocks using a microtome and mounted on glass slides. Staining: Sections are stained with dyes such as Hematoxylin and Eosin (H&E) to differentiate cellular components and structures.
