The creation of histological datasets involves several steps:
1. Sample Collection: Tissue samples are collected from patients or experimental models, ensuring proper preservation to maintain tissue integrity.
2. Tissue Processing: Samples are processed through fixation, embedding, sectioning, and staining to prepare them for microscopic examination.
3. Imaging: High-resolution images of the prepared tissue sections are captured using appropriate microscopy techniques.
4. Annotation and Analysis: Images are annotated by experts to identify and label relevant features. Various image analysis software tools may be used to extract quantitative data from the images.
