Histochemical techniques are applied in several steps:
Fixation: Tissue samples are preserved using fixatives like formalin to maintain cellular structure and prevent degradation. Embedding: Fixed tissues are embedded in a medium, usually paraffin, to facilitate sectioning. Sectioning: Thin sections of the tissue are cut using a microtome and placed on slides. Staining: The sections are stained using specific histochemical techniques to highlight particular cellular components. Microscopy: Stained slides are examined under a microscope to study the tissue architecture and identify any pathological changes.
