Various techniques are employed to study expression patterns. Some of the most commonly used methods include:
Immunohistochemistry (IHC): This technique uses antibodies to detect specific proteins in tissue sections, allowing visualization of protein expression. In Situ Hybridization (ISH): This method uses labeled RNA or DNA probes to detect specific nucleic acid sequences in tissue sections, providing information on gene expression. Real-Time PCR (qPCR): This technique quantifies mRNA levels in tissues, offering a measure of gene expression. RNA Sequencing (RNA-seq): This high-throughput method sequences RNA to provide a comprehensive view of gene expression across tissues.
