Identifying driver mutations involves a combination of genetic sequencing, bioinformatics analysis, and functional studies. High-throughput sequencing technologies, such as next-generation sequencing (NGS), are often employed to detect mutations across the genome. Bioinformatics tools then distinguish between driver and passenger mutations by evaluating the frequency and functional impact of these mutations. Functional assays, including cell culture and animal models, help confirm the role of specific mutations in cancer development.
