How are Cysteine Proteases Detected in Histological Samples?
The detection of cysteine proteases in histological samples can be performed using various techniques. Immunohistochemistry (IHC) is commonly used to localize these enzymes within tissues using specific antibodies. Enzyme activity assays and Western blotting can also be employed to measure the presence and activity levels of cysteine proteases. Additionally, molecular techniques such as PCR can be used to quantify mRNA levels of these enzymes, providing insight into their expression patterns.
