Histological examination of connective tissues involves several steps: - Fixation: Tissues are preserved using fixatives such as formalin to prevent degradation. - Embedding and Sectioning: Fixed tissues are embedded in paraffin and sectioned into thin slices using a microtome. - Staining: Stains like Hematoxylin and Eosin (H&E), Masson's trichrome, and others are used to differentiate various components of connective tissues. - Microscopy: Stained sections are examined under a microscope to study the tissue architecture and identify abnormalities.
