How are Cholinergic Neurons Identified in Histology?
In histological studies, cholinergic neurons can be identified through various staining techniques. Immunohistochemistry is commonly used, employing antibodies that target enzymes involved in acetylcholine synthesis, such as choline acetyltransferase (ChAT). Acetylcholinesterase staining can also be utilized to identify regions with high cholinergic activity.