Establishing a cell culture involves several steps:
Tissue Extraction: Cells are extracted from the tissue of interest using mechanical or enzymatic methods. Medium Preparation: Cells are placed in a nutrient-rich medium that supports their growth. The medium composition can vary depending on the cell type. Incubation: The culture is incubated under controlled conditions (temperature, humidity, CO2) to promote cell growth and division. Sub-culturing: As cells proliferate, they may need to be transferred to new vessels to prevent overcrowding and maintain optimal growth conditions.
