Several factors influence the rate of reagent penetration into tissue samples:
Tissue Size and Thickness: Smaller and thinner sections allow for quicker and more uniform penetration. Reagent Composition: The molecular size and polarity of reagents can affect their diffusion rates. Temperature: Increasing the temperature can enhance the diffusion rate of reagents into tissues. Agitation: Gentle shaking or stirring can promote more uniform penetration of reagents. Vacuum Infiltration: Using a vacuum can remove air bubbles and facilitate faster reagent infiltration.
