While cell culture systems offer valuable insights, they come with challenges:
Contamination: Cultures can be contaminated with bacteria, fungi, or other cell lines, compromising the results. Cell Line Authenticity: Ensuring that cell lines are correctly identified and free from cross-contamination is critical. Reproducibility: Variability in culture conditions can lead to inconsistent results. Ethical Considerations: The use of human and animal tissues for cell culture raises ethical concerns that must be addressed.
