The preparation of cell culture models involves several key steps:
Isolation: Cells are isolated from tissues using enzymatic digestion or mechanical dissociation. The choice of method depends on the tissue type and the desired cell population. Culturing: Isolated cells are cultured in appropriate growth media under controlled conditions (temperature, CO2, humidity). The media composition varies depending on the cell type and experimental requirements. Subculturing: Cells are periodically passaged to prevent overcrowding and to maintain their viability. This involves detaching cells from the culture surface and redistributing them into new culture vessels.
