What are the Limitations of Using EEA1 as a Marker?
While EEA1 is a valuable marker for early endosomes, there are limitations to its use. For instance, the specificity of antibodies can sometimes be an issue, leading to non-specific binding and false-positive results. Additionally, the dynamic nature of endosomes means that EEA1 localization can vary depending on the cell type and physiological conditions. Therefore, it is essential to use appropriate controls and complementary markers to validate findings.
