The preparation of acrylic resins for embedding involves several steps:
Dehydration: The tissue is dehydrated using a series of graded alcohols to remove water. Infiltration: The dehydrated tissue is infiltrated with the acrylic resin, often under vacuum, to ensure complete penetration. Polymerization: The resin is polymerized, typically using heat or UV light, to harden and embed the tissue. Sectioning: The embedded tissue is sectioned using a microtome, producing thin slices for microscopic examination.