Preparing Tris buffers involves dissolving Tris base in water and adjusting the pH with hydrochloric acid (HCl) or sodium hydroxide (NaOH). For example, to prepare a 1M Tris buffer at pH 7.5, one would:
Dissolve 121.14 g of Tris base in 800 mL of distilled water. Adjust the pH to 7.5 using concentrated HCl. Bring the final volume to 1 liter with distilled water.
It is crucial to check the pH after dilution, as the pH can change with temperature and concentration.
