RT-qPCR is employed in histology for several reasons:
Sensitivity and Specificity: RT-qPCR can detect low-abundance transcripts, providing precise quantification of gene expression. Quantitative Data: Unlike traditional PCR, RT-qPCR provides quantitative data, allowing for the comparison of expression levels between different samples. Validation: It is often used to validate results obtained from other techniques like microarray analysis and RNA-seq.
