In histological studies, safranin is used because it binds to acidic tissue components, particularly those rich in nucleic acids, such as nuclei and chromatin. This property makes it an excellent counterstain, particularly in protocols where multiple stains are used to differentiate various tissue types and structures. Safranin is often used in combination with other dyes, such as fast green or hematoxylin, to provide contrast and enhance the visualization of different cellular components.
