Pore size is paramount for several reasons: 1. Tissue Permeability: It affects the permeability of tissues, which is crucial for nutrient and waste exchange. 2. Staining Techniques: It influences the penetration and distribution of histological stains and dyes. 3. Sample Preparation: Appropriate pore size ensures effective fixation, dehydration, and embedding of tissue samples. 4. Synthetic Scaffolds: In tissue engineering, the pore size of scaffolds affects cell migration, proliferation, and differentiation.