In histology, the ability to observe unstained, living tissues is crucial for understanding cellular processes and dynamics. Phase contrast microscopy provides several advantages:
Visualization of Living Cells: It allows for the examination of living cells and tissues without the need for staining, which can alter cell behavior or even be toxic. Enhanced Detail: The technique enhances the visibility of structures within cells, such as nuclei, vacuoles, and organelles, providing greater detail and contrast. Non-destructive: Since no staining is required, the cells and tissues remain viable for further experiments or observations.
