There are multiple reasons why color distortion might occur in histology. Some of the primary causes include:
Staining Techniques: Different staining methods produce distinct colors, and improper application can lead to inconsistent or misleading results. Fixation: The process of preserving tissue samples can affect the uptake of stains, leading to color changes. Section Thickness: Variations in the thickness of tissue sections can result in differential staining and subsequently color distortion. Microscope Settings: The settings on the microscope, including light intensity and filter selection, can alter the perceived colors. Digital Imaging: The conversion of microscopic images to digital formats can introduce color inaccuracies due to differences in camera sensors and display screens.
