Choosing the right objective depends on the specific requirements of the histological examination. Factors to consider include:
Magnification: Objectives come in various magnifications, typically ranging from 4x to 100x. Higher magnifications are necessary for observing fine cellular details. Numerical Aperture (NA): The NA indicates the objective's ability to gather light and resolve fine specimen detail. Higher NA values provide better resolution. Working Distance: This is the distance between the objective lens and the specimen. Longer working distances are beneficial for thicker specimens. Correction for Aberrations: Depending on the level of detail required, one might choose achromatic, plan, or apochromatic objectives.