Cell Viability: Over time, cells can deplete the nutrients in their growth medium and produce waste products. Regular passages help replenish the nutrients and remove waste. Cell Density: Maintaining an optimal cell density is essential for healthy cell growth. High density can lead to contact inhibition, where cells stop growing due to lack of space. Genetic Stability: Frequent passages can help minimize the risk of genetic drift, which can alter the characteristics of the cell line over time.
