In Histology, the study of the microscopic anatomy of cells and tissues, microscopy is an essential tool. ND filters help in controlling the intensity of the light source in a microscope, which is critical for several reasons:
Preventing Photobleaching: Intense light can cause photobleaching of fluorescent dyes used in staining, leading to loss of signal and poor image quality. Enhancing Contrast: By reducing light intensity, ND filters can enhance the contrast of the specimen, making it easier to observe fine details. Protecting Specimens: Excessive light can damage sensitive biological specimens, so ND filters help in protecting samples during prolonged observations.