Buffers are essential in histology for several reasons:
1. Staining Consistency: Many histological stains are pH-sensitive. Buffers ensure that the pH remains stable, providing consistent and accurate staining results. 2. Tissue Preservation: During fixation, buffers help maintain the natural pH of tissues, preventing autolysis and degradation. 3. Optimal Enzyme Activity: Enzymatic reactions, such as those involved in certain staining techniques, require specific pH ranges to function correctly.
