Several cutting-edge technologies facilitate real time visualization in histology:
Confocal Microscopy: This technique uses laser light to scan specimens, providing high-resolution images with the ability to focus on different depths within the tissue. Multiphoton Microscopy: Utilizing longer wavelength light, this method allows for deeper tissue penetration and reduced phototoxicity, making it ideal for live tissue imaging. Light Sheet Fluorescence Microscopy: This approach illuminates a thin sheet of tissue, capturing high-speed, high-resolution images in three dimensions. Optical Coherence Tomography (OCT): OCT offers cross-sectional imaging of tissues with micrometer resolution, similar to ultrasound but using light waves instead of sound waves. Fluorescence Lifetime Imaging Microscopy (FLIM): FLIM measures the decay rate of fluorescence from a sample, providing information about the molecular environment within tissues.
