Several advanced techniques in histology and related fields utilize carbon 13: - Magnetic Resonance Spectroscopy (MRS): This technique exploits the magnetic properties of 13C to provide detailed information about the chemical environment of carbon atoms in tissues. - Mass Spectrometry: By measuring the mass-to-charge ratio of ions, mass spectrometry can identify and quantify 13C-labeled compounds, offering precise insights into metabolic pathways. - Stable Isotope Probing (SIP): This method involves feeding organisms with 13C-labeled substrates and then tracking the incorporation of 13C into cellular biomolecules, helping to elucidate metabolic activities.
