Clinical histologists employ a variety of techniques to prepare and analyze tissue samples. Some of the widely used methods include:
Histochemistry: Involves the use of chemical reactions to identify and visualize specific tissue components. Immunohistochemistry (IHC): Uses antibodies to detect specific proteins within tissues, aiding in the diagnosis of diseases like cancer. In situ hybridization (ISH): A technique used to locate specific nucleic acid sequences within tissue sections. Electron microscopy: Provides detailed images of tissue ultrastructure, essential for diagnosing certain diseases. Frozen sectioning: A rapid technique used during surgeries to provide immediate pathological assessment.
