What Techniques are Used to Study RUNX2 in Histology?
Several histological techniques are employed to study RUNX2 expression and function. These include immunohistochemistry (IHC), which uses specific antibodies to detect RUNX2 protein in tissue sections, and in situ hybridization (ISH) to visualize RUNX2 mRNA expression. Additionally, molecular techniques such as quantitative PCR and Western blotting are used to quantify RUNX2 levels in various tissues and cells.