Several techniques are used to study pre-mRNA and its processing:
- RT-PCR (Reverse Transcription Polymerase Chain Reaction): This technique converts RNA into cDNA and amplifies specific sequences to study gene expression. - Northern Blotting: This method detects specific RNA sequences in a sample, allowing researchers to study the abundance and size of mRNA. - In Situ Hybridization: This technique uses labeled probes to visualize the location of specific RNA sequences within tissue sections.
