mrna secondary structure

What Techniques are Used to Study mRNA Secondary Structures?

Several techniques are employed to study mRNA secondary structures, including:
SHAPE (Selective 2'-Hydroxyl Acylation analyzed by Primer Extension)
NMR spectroscopy (Nuclear Magnetic Resonance)
X-ray crystallography
Cryo-EM (Cryogenic Electron Microscopy)
Computational prediction tools such as RNAfold

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