What Techniques are Used to Study miRNAs in Histology?
Several techniques are used to study miRNAs in histology. These include:
- In situ hybridization (ISH): This technique allows for the visualization of miRNA expression within tissue sections. - Quantitative PCR (qPCR): Used to quantify miRNA levels in tissues. - Next-Generation Sequencing (NGS): Provides a comprehensive analysis of miRNA expression profiles. - Microarray Analysis: Used to profile miRNA expression across different tissue types or conditions. - Immunohistochemistry (IHC): Though more commonly used for proteins, can be adapted to study the effects of miRNAs on protein expression.
