What Techniques are Used to Study FGF23 in Histology?
Several techniques are employed to study FGF23 in histology:
Immunohistochemistry (IHC): This technique uses antibodies to detect FGF23 in tissue sections, allowing for localization and quantification. In Situ Hybridization (ISH): ISH helps visualize the mRNA expression of FGF23, providing insights into its gene regulation. Western Blotting: This method detects FGF23 protein levels in tissue extracts, aiding in the study of its expression and function.