What Techniques are Used to Identify Biological Agents in Tissue Samples?
Several histological techniques are employed to identify biological agents in tissue samples:
Histochemical Staining: Techniques such as Gram staining for bacteria and PAS staining for fungi help in visualizing these agents. Immunohistochemistry: This involves the use of antibodies to detect specific antigens in tissues, aiding in the identification of viruses and bacteria. Electron Microscopy: This provides high-resolution images that can reveal the ultrastructure of viruses and bacteria. Molecular Techniques: Techniques like PCR and in situ hybridization can detect the genetic material of pathogens.
