What Techniques Are Used to Identify Antibiotic Resistance Genes in Histological Samples?
Several techniques are employed to identify antibiotic resistance genes in histological samples:
In Situ Hybridization (ISH): This technique uses labeled probes to detect specific DNA or RNA sequences within tissue sections. Polymerase Chain Reaction (PCR): PCR can amplify specific antibiotic resistance genes from extracted DNA, making them easier to identify. Immunohistochemistry (IHC): This method uses antibodies to detect proteins encoded by antibiotic resistance genes. Fluorescence In Situ Hybridization (FISH): FISH combines fluorescent probes with hybridization techniques to visualize the presence of resistance genes.
