What Techniques are Used to Detect Molecular Markers?
Several techniques are employed to detect and analyze molecular markers:
1. Immunohistochemistry (IHC): Uses antibodies to detect specific proteins in tissue sections. 2. In Situ Hybridization (ISH): Detects specific nucleic acid sequences within tissue samples. 3. Fluorescence In Situ Hybridization (FISH): Uses fluorescent probes to identify specific DNA or RNA sequences. 4. Polymerase Chain Reaction (PCR): Amplifies DNA sequences to detect specific genetic markers. 5. Western Blotting: Identifies specific proteins from a tissue extract.
