What Techniques are Used in Histology to Study Muscular Diseases?
Several specialized techniques are employed in histology to study muscular diseases:
Hematoxylin and Eosin (H&E) Staining: This is the most common staining method used to evaluate general muscle architecture and the presence of inflammatory cells. Immunohistochemistry (IHC): IHC is used to detect specific proteins within muscle tissue, such as dystrophin in cases of DMD. It can also identify immune cells in inflammatory myopathies. Enzyme Histochemistry: This technique helps to assess enzyme activity in muscle fibers, which can be altered in metabolic myopathies. Electron Microscopy: Provides ultrastructural details, useful for identifying rimmed vacuoles in IBM and other submicroscopic changes.
