Several techniques are employed in histology research, each with its unique applications and advantages.
- Light Microscopy: This is the most basic and widely used technique, where tissues are stained with specific dyes to highlight different cellular components. - Electron Microscopy: Provides much higher resolution images than light microscopy, allowing for the visualization of ultrastructural details. - Immunohistochemistry (IHC): Uses antibodies to detect specific proteins in tissue sections, aiding in the identification and localization of cellular components. - In Situ Hybridization (ISH): Detects specific nucleic acid sequences within tissues, useful for identifying the presence of particular genes or mRNA. - Histochemical Staining: Uses chemical reactions to visualize specific tissue components, such as glycogen, lipids, or enzymes.
