What Techniques Are Used in Histology for Monitoring Treatment Response?
Several techniques are employed in histology to monitor treatment response, including:
Hematoxylin and Eosin (H&E) staining: This basic staining technique helps visualize the general tissue structure and identify abnormal cells. Immunohistochemistry (IHC): This method uses antibodies to detect specific proteins or antigens in tissues, helping to identify cellular changes and the presence of therapeutic targets. In situ hybridization (ISH): This technique detects specific DNA or RNA sequences in tissues, providing insights into gene expression and genetic alterations. Electron microscopy: This advanced technique offers high-resolution images of cellular and subcellular structures, allowing for detailed analysis of treatment effects at the ultrastructural level.
