Several techniques can be employed to ensure a gentle approach:
1. Tissue Fixation: Proper fixation is crucial. Using mild fixatives like formalin or glutaraldehyde in appropriate concentrations can help preserve tissue without causing shrinkage or hardening. 2. Sectioning: Using sharp microtome blades and adjusting the cutting speed can minimize tearing and compression artifacts. 3. Staining: Gentle staining protocols that avoid overexposure to harsh chemicals can help in preserving the tissue morphology. 4. Mounting: Using low-viscosity mounting media and avoiding air bubbles can prevent distortion of the tissue sections.
