What Are the Steps Involved in Verhoeff's Staining?
The procedure for performing Verhoeff's stain involves several steps:
Fixation: Tissue samples are fixed using formalin or another appropriate fixative. Dehydration: Samples are dehydrated through a series of alcohol washes. Staining: The tissue is stained with a mixture of hematoxylin, ferric chloride, and iodine. Differentiation: Excess stain is removed using a solution of ferric chloride and ethanol. Counterstaining: A secondary stain, such as Van Gieson's, is applied to highlight other tissue components. Dehydration and Clearing: The samples are dehydrated again and cleared using xylene. Mounting: Finally, the samples are mounted on slides with a coverslip.
