What Techniques are Used for 3D Imaging in Histology?
Several techniques are employed for 3D imaging in histology, including:
Confocal Microscopy: Uses point illumination and a spatial pinhole to eliminate out-of-focus light, providing high-resolution images at different depths, which are then reconstructed into 3D images. Multiphoton Microscopy: Similar to confocal microscopy but uses longer wavelengths, allowing for deeper tissue penetration and reduced phototoxicity. Optical Coherence Tomography: Uses light waves to capture micrometer-resolution, cross-sectional images of tissues. Light Sheet Fluorescence Microscopy: Illuminates a thin sheet of the sample, allowing for fast and high-resolution imaging of large specimens. Micro-CT and MRI: Provide high-resolution images of tissue structure and composition in a non-destructive manner.
