Sample handling in histology involves several critical steps, each aimed at preserving the structure and composition of the tissue. Key steps include:
1. Collection: Tissue samples are collected using sterile instruments to prevent contamination. 2. Fixation: Samples are immediately placed in a fixative, commonly formalin, to preserve tissue architecture and prevent autolysis. 3. Transport: Fixed samples are securely transported to the histology lab, maintaining a stable environment to avoid any physical or chemical alterations. 4. Processing: Samples undergo dehydration, clearing, and infiltration with paraffin to prepare them for sectioning. 5. Embedding: Samples are embedded in paraffin blocks to provide support for thin sectioning. 6. Sectioning: Thin sections are cut using a microtome and placed on glass slides. 7. Staining: Sections are stained to highlight different tissue components, commonly using Hematoxylin and Eosin (H&E).
