What role do advanced microscopy techniques play in modern histology?
Traditional light microscopy has its limitations, particularly in terms of resolution and depth of field. Modern histology has seen the adoption of advanced microscopy techniques such as confocal microscopy and multiphoton microscopy. These methods provide high-resolution, three-dimensional images of tissues, allowing for a more detailed analysis of cellular structures and interactions.
Another noteworthy development is the use of super-resolution microscopy techniques like STED (Stimulated Emission Depletion) and PALM (Photoactivated Localization Microscopy). These techniques surpass the diffraction limit of light, offering unprecedented resolution that can reveal molecular details at the nanoscale.
