Fixation: The tissue sample is fixed using an appropriate fixative such as formalin to preserve tissue architecture. Deparaffinization and Hydration: If the sample is embedded in paraffin, it is deparaffinized and rehydrated through a series of alcohol baths. Staining: The sample is treated with a staining solution containing potassium ferricyanide and hydrochloric acid to initiate the reaction with ferrous iron. Counterstaining (optional): A counterstain such as nuclear fast red may be used to provide contrast and highlight cellular structures. Dehydration and Mounting: The sample is dehydrated, cleared, and mounted for microscopy.
