The general procedure for Sirius Red Staining involves the following steps:
Fixation of the tissue sample, commonly using formalin. Embedding the tissue in paraffin and sectioning it into thin slices. Deparaffinization and hydration of the tissue sections. Staining with Sirius Red dye, often in a saturated aqueous solution. Washing and counterstaining, usually with hematoxylin. Dehydration, clearing, and mounting of the stained sections.
Each step must be carefully controlled to ensure optimal staining and accurate results.
