What is Electrophoretic Mobility Shift Assay (EMSA)?
The Electrophoretic Mobility Shift Assay (EMSA) is a powerful technique used to study protein-DNA interactions. It is based on the principle that the migration of a DNA or RNA molecule through a polyacrylamide or agarose gel will slow down when it is bound to a protein, due to the increase in size and change in charge of the complex.