Once the tissue sample is collected, it undergoes several steps:
- Fixation: Preserving the tissue to prevent decay and maintain its structural integrity. - Embedding: Placing the tissue in a medium like paraffin to provide support for thin sectioning. - Sectioning: Cutting thin slices of the embedded tissue using a microtome. - Staining: Applying specific dyes to highlight different cellular components. Common stains include Hematoxylin and Eosin (H&E).